User Manuals
Single Cell
| User Manual Name (click to download) | Description | Sequencing Technology | Species | Product Catalog Number |
|---|---|---|---|---|
| Single Cell RNA Boost Kit with panels | Describes how to remove ~50% uninformative human reads that are not used in downstream analysis and redistribute the reads to the transcriptome from 10x Chromium Next-GEM Single Cell libraries. | Short read | Human | KIT1036, KIT1022, KIT1024, KIT1025 |
| Single Cell RNA Boost Kit with PacBio MAS-Seq | Describes how to use Single Cell RNA Boost to deplete the single-cell cDNA material generated from the 10x Chromium Next GEM Single Cell 3’ Kit v3.1 in order to prepare inputs for the PacBio MAS-Seq for 10x Single Cell 3′ Kit and PacBio sequencing. | PacBio Sequel II/Iie and Revio systems | Human | KIT1036, KIT1022, KIT1024, KIT1025 |
Mito DNA Depletion Kit for the BD Rhapsody Whole Transcriptome Analysis (WTA) Assay | Describes how to use Mito DNA depletion with BD Rhapsody whole transcriptomic workflow to improve single cell data | BD Rhapsory | Human | KIT2002 |
Bulk RNA and DNA Depletion
| User Manual Name (click to download) | Description | Sequencing Technology | Species | Product Catalog Number |
|---|---|---|---|---|
| Stranded total RNA prep with depletion | Describes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat rRNA and/or high expressing gene panels. | Short read RNAseq | Human, Mouse, Rat | KIT1014, KIT1022, KIT1024, KIT1025, KIT1027 |
| Plus Stranded Total RNA Prep with Depletion | Describes how to prepare and deplete stranded RNA-Seq libraries from total RNA. Depletion removes overabundant human, mouse, rat, and 212 bacteria rRNA. | Short read RNAseq | Human, Mouse, Rat, Bacteria | KIT1016 |
| Unique Dual Index Adapter Plate for RNA Prep (Set A) | This spreadsheet lists the P5 and P7 adapter sequences for use. | Short read RNASeq | n/a | KIT1014, KIT1016, KIT1017 |
| Post-library multiplex ribodepletion (HMR) | Describes how to remove overabundant human, mouse, rat rRNA from prepared RNA-Seq libraries to improve sequencing sensitivity and performance at a high-throughput scale. | Short read RNASeq | Human, Mouse, Rat | KIT1026, KIT3000s |
| Post-library multiplex ribodepletion (HMR Plus) | Describes how to remove overabundant human, mouse, rat, or bacterial-species rRNA from prepared RNA-Seq libraries to improve sequencing sensitivity and performance at a high-throughput scale. | Shot read RNA | Human, Mouse, Rat, pan-bacterial | KIT1030 |
| High Expressing Gene Depletion | Describes how to remove highly expressed RNA from whole transcriptome to improve sensitivity of low-frequency variants | Short read RNA | Human | KIT1020 |
| Mitochondrial DNA Depletion | Describes how to remove mitochondrial DNA from prepared DNA sequencing libraries | Short read DNA | Human | KIT2002 |
| Nasopharyngeal RNA Depeltion | Describes how to prepare strand-specific RNA libraries depleted of human and bacterial ribosomal RNA (rRNA) and abundant human nasopharyngeal mRNA for sequencing on Illumina® instruments | Short read RNA | Human | KIT1039 |
| Human DNA Depletion | Describes how to remove human DNA from samples with high human content and reassign sequencing reads to microbial content | Short read DNA | Human | KIT2020 |